| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207526 |
| Chromosome: | chromosome 9 |
| Location: | 5644315 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401145 | (1 of 2) 3.1.4.12 - Sphingomyelin phosphodiesterase / Neutral sphingomyelinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCATGGTGTCACTTGCTGCTTGCTGAG |
| Internal bar code: | CCTATCGGGCGCCTCCGGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 98.4045 |
| LEAP-Seq n confirming: | 15049 |
| LEAP-Seq n nonconfirming: | 244 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCACGCAGCAATACCA |
| Suggested primer 2: | AAGTCTGTTTCCGTGATGGG |