| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207532 |
| Chromosome: | chromosome 11 |
| Location: | 1296473 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467711 | (1 of 3) PTHR30612 - SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGTGCATGCATGCGCCTGTGCCCAGTC |
| Internal bar code: | AACAAGCGTCGTGTTCACAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 99.883 |
| LEAP-Seq n confirming: | 854 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCGTAGGCATAATGCTG |
| Suggested primer 2: | CTACCGGTGCGTTGGTTATT |