Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.207547 |
Chromosome: | chromosome_5 |
Location: | 3264063 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g240225 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGCGGTTCAACACCGTGAAGCAGTGGTCG |
Internal bar code: | GGAGAGCAGCGTCACGCTACAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 585 |
LEAP-Seq percent confirming: | 99.1303 |
LEAP-Seq n confirming: | 19491 |
LEAP-Seq n nonconfirming: | 171 |
LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCACCTATTCGATCCCTAA |
Suggested primer 2: | CTTGACGCAGATGGCATAGA |