| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207557 |
| Chromosome: | chromosome 1 |
| Location: | 2228068 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012200 | (1 of 3) PF00651//PF03110 - BTB/POZ domain (BTB) // SBP domain (SBP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAACTGAGCAGTCAGCTGTCGTGGGTT |
| Internal bar code: | AACGCGGTGTTGAGAGCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 97.0315 |
| LEAP-Seq n confirming: | 523 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCACTTCGTTCCGTCCAT |
| Suggested primer 2: | TCGAGCCCTTCTGTGTTTCT |