| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207602 |
| Chromosome: | chromosome 12 |
| Location: | 3508301 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512950 | CIT1 | (1 of 1) K07240 - chromate transporter (chrA); Chromate ion transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCGACACCCACCCACGCACCCACAAT |
| Internal bar code: | GGTCATTCTAGGGAAGGTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.8322 |
| LEAP-Seq n confirming: | 1785 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGGCCCTCAACTCCTTG |
| Suggested primer 2: | GGATGCCATACCGTAGTGCT |