Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.207618 |
Chromosome: | chromosome 16 |
Location: | 2893677 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g663750 | (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCTCTCGGCCCGCCAGCCCAAGGCATA |
Internal bar code: | GGTGGGTCGATTGCTTTGGACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 261 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 883 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCATTCAAGTGCAGTGAGGC |
Suggested primer 2: | GCAAGAACACAAAGCAGCAC |