| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207628 |
| Chromosome: | chromosome 6 |
| Location: | 2497511 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268976 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCATTACTACCCGTTTCGAAGGAAGTC |
| Internal bar code: | CGAACATTGCTGCTGAGAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 99.7114 |
| LEAP-Seq n confirming: | 5873 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCCACATCTTGTTACTT |
| Suggested primer 2: | CAGATGCGCGTAATTCTCAA |