| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.207651 |
| Chromosome: | chromosome_6 |
| Location: | 2791235 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g271150 | FAP74 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCAAGGTGCGGTGCGGTGTGATGCGGTGT |
| Internal bar code: | GGACTCACTAAATGCCTTACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 96.2602 |
| LEAP-Seq n confirming: | 1776 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCTGGGCTGAACTCGAA |
| Suggested primer 2: | GAACACAACAACATTCCCCC |