| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207656 |
| Chromosome: | chromosome_9 |
| Location: | 1683632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g397000 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCGATCCCTGCAGAGGCACGAATACACTA |
| Internal bar code: | GGCTGACACCTACGACCTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 88.6792 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCAGCAACTCAGACAA |
| Suggested primer 2: | AGTACGGTACACCCACCTGC |