| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207657 |
| Chromosome: | chromosome 16 |
| Location: | 1848189 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCGCTTGACAAGATGCTGGAGTGTCGA |
| Internal bar code: | CCGGGAGAAACGGAGTAGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 959 |
| LEAP-Seq percent confirming: | 99.3281 |
| LEAP-Seq n confirming: | 4139 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTCGGTAACAAGACCAG |
| Suggested primer 2: | AACGCAAGGTATATCGGCAC |