| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207660 |
| Chromosome: | chromosome_4 |
| Location: | 3491352 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g227900 | PF20 | WD-repeat protein of flagellum central pair | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTATTTGCCTCCCCATCCGCCACAATCTCT |
| Internal bar code: | GGGGCGCTGCCCCGCGGTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 90.1646 |
| LEAP-Seq n confirming: | 2081 |
| LEAP-Seq n nonconfirming: | 227 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTAAGGGGAAGAACGAT |
| Suggested primer 2: | GGTCGTTTCTTGCGATTCAA |