| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207661 |
| Chromosome: | chromosome_6 |
| Location: | 5328976 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g283250 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCTCCACGCTCTTATCGTGTACCGCCGCC |
| Internal bar code: | TCAGGATCGAACGACGTCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 91.952 |
| LEAP-Seq n confirming: | 36984 |
| LEAP-Seq n nonconfirming: | 3237 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACTTCTTCTGTTTTCG |
| Suggested primer 2: | CGATACCCACCATTTTACCG |