| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207661 |
| Chromosome: | chromosome 6 |
| Location: | 5328976 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283250 | POB9 | Proteome of basal body 9 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTATAGCGGCGGTGGCAGCGGGGGCGGC |
| Internal bar code: | TTTTTGCGTATACGTCTGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.1821 |
| LEAP-Seq n confirming: | 8973 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACTTCTTCTGTTTTCG |
| Suggested primer 2: | CGATACCCACCATTTTACCG |