| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207663 |
| Chromosome: | chromosome 10 |
| Location: | 6346056 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465450 | (1 of 2) 2.8.2.20 - Protein-tyrosine sulfotransferase / Tyrosylprotein sulfotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCCCGCCGCCCGCGCGGCTCCTCCCCA |
| Internal bar code: | TTACCGTGCAGTGAACGTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 26 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGTAGCGGCAGTAGCA |
| Suggested primer 2: | GTGCACACATGTAGGCATCC |