| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207708 |
| Chromosome: | chromosome 16 |
| Location: | 7525147 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687966 | FAP5,Nphp3 | Flagellar Associated Protein 5; (1 of 2) PTHR19959//PTHR19959:SF147 - KINESIN LIGHT CHAIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACACACCGCCCATCTTTTCCCTTCACCA |
| Internal bar code: | AAAACATGGCCCTTATACAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 99.7817 |
| LEAP-Seq n confirming: | 457 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTACAGGCGATGATCC |
| Suggested primer 2: | GGGGACAGAGGTTATGGGAT |