| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207730 |
| Chromosome: | chromosome 10 |
| Location: | 900961 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424200 | POLE2 | (1 of 1) K02325 - DNA polymerase epsilon subunit 2 (POLE2); DNA polymerase epsilon subunit 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTCTGCATGTGCAAAGCATAACTCTGT |
| Internal bar code: | TGAGTTTCTGAGTATGACAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 98.6304 |
| LEAP-Seq n confirming: | 4393 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGAGGAGGAGGAGGAG |
| Suggested primer 2: | GGGTTACGTGAGCTGGATGT |