Insertion junction: LMJ.RY0402.207731_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.207731
Chromosome:chromosome 3
Location:5899926
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g190000 PPP14 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGTGAACCGCTCGGCAGACTGCTCCGTA
Internal bar code:CCCTTCTACGTGACAAAAGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1013
LEAP-Seq percent confirming:99.4486
LEAP-Seq n confirming:1984
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTTAGCGGTCAAGAGGGA
Suggested primer 2:AGTGATGAGGGACGGATGAG