| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.207744 |
| Chromosome: | chromosome 4 |
| Location: | 3610620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228625 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGACTATACGTAATATACGCTGTAATG |
| Internal bar code: | ACAAGAATTGTGGAGTGCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 122 |
| LEAP-Seq percent confirming: | 99.3225 |
| LEAP-Seq n confirming: | 733 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACAGCACATTCCCCCTG |
| Suggested primer 2: | ATGCATGTGGAGATTGTGGA |