| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207745 |
| Chromosome: | chromosome 14 |
| Location: | 975553 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614000 | CGL17 | Conserved in the Green Lineage; (1 of 1) PTHR35699:SF1 - F2J10.10 PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAGCTGCCGTCTGCAAGAGCAGAGAGG |
| Internal bar code: | GTCGCTGAGAATGGGTAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 99.451 |
| LEAP-Seq n confirming: | 3804 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGGTTGTAACAGGGAAT |
| Suggested primer 2: | GCCCTTCCCAACTTTACACA |