Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.207755 |
Chromosome: | chromosome_8 |
Location: | 1053329 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362100 | FAL11 | Similar to Flagellar Associated Protein FAP154 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACACCTGCGTGCGTCACCTGCAGTCGTAG |
Internal bar code: | TGGTAACCGACATCTGGAATAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 905 |
LEAP-Seq percent confirming: | 88.5167 |
LEAP-Seq n confirming: | 185 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCTGACTTCAGTAGGGGCA |
Suggested primer 2: | TCTGTGAGGGCGTCTTCTTT |