| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207776 |
| Chromosome: | chromosome 14 |
| Location: | 295118 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609750 | (1 of 6) PF03351 - DOMON domain (DOMON) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGATGGCCGGAGGAACCCAGAGCCACT |
| Internal bar code: | GAAGGATGGGTTCCGCGCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 803 |
| LEAP-Seq percent confirming: | 99.7826 |
| LEAP-Seq n confirming: | 1377 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCCACTACTGACACAAGC |
| Suggested primer 2: | GTGCTCGTTCTCAGACTCCC |