Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.207840 |
Chromosome: | chromosome_2 |
Location: | 104574 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g074000 | POC18 | Centriole proteome protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCCTGTCCCGCACCTCAGCTGCCTGCTGGG |
Internal bar code: | CCGAGTCCATGTAACCAGCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 915 |
LEAP-Seq percent confirming: | 89.0962 |
LEAP-Seq n confirming: | 5254 |
LEAP-Seq n nonconfirming: | 643 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGTGGCAGTAAGACCGCT |
Suggested primer 2: | AGCGCCTTCTTATTGTGGAA |