Insertion junction: LMJ.RY0402.207840_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.207840
Chromosome:chromosome 3
Location:8923098
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g208497 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACCTGTGATGCTCACACTGTGCTGAGAG
Internal bar code:AGGACACGTGACCGCTTTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:625
LEAP-Seq percent confirming:99.4007
LEAP-Seq n confirming:1161
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGTGGTAGACGGTAGGGG
Suggested primer 2:CTCGTGAGAGATCCTGCCTC