| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207883 |
| Chromosome: | chromosome 12 |
| Location: | 3986115 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516750 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGCAGCGATAGTTCGCGTCTGTTTGCA |
| Internal bar code: | GGGGCAGGCCACGCCAGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 95.328 |
| LEAP-Seq n confirming: | 1918 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCACGGAACAGTAGTGGC |
| Suggested primer 2: | AGTGTCACAACCCCCTTGAG |