| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207897 |
| Chromosome: | chromosome 11 |
| Location: | 2460520 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475550 | UMM10 | (1 of 2) 2.1.1.165 - Methyl halide transferase / Methyl chloride transferase; methyl-halide synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCCCGGATCCAACAGTAATAGCGCAC |
| Internal bar code: | CCGGGACGTATCGAGGGCCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 97.0012 |
| LEAP-Seq n confirming: | 1682 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACCGGTGTACGATACGA |
| Suggested primer 2: | AATAGGGATGTCAGGATGCG |