| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207935 |
| Chromosome: | chromosome 2 |
| Location: | 4562043 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100800 | (1 of 1) PTHR22847//PTHR22847:SF410 - WD40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 27 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCAGCACCCCGCTCGGTGCTGCCCGGG |
| Internal bar code: | GGCGCATAGGGCGTCAATAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 99.1293 |
| LEAP-Seq n confirming: | 3757 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAATGCCTTTCATTGCACG |
| Suggested primer 2: | CACACACACACACAATCCCA |