| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207936 |
| Chromosome: | chromosome_14 |
| Location: | 3759925 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g632350 | FAP138 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGGCCCGGTGCTGCGAGTGGTACGGGCAT |
| Internal bar code: | TGAGGATGGGTGTAGCCAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 99.7778 |
| LEAP-Seq n confirming: | 1347 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGATATTGGTGCAGCCC |
| Suggested primer 2: | ACACGGTTTCACTCACTCCC |