| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207939 |
| Chromosome: | chromosome 16 |
| Location: | 969298 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648750 | (1 of 1) PF10373 - Est1 DNA/RNA binding domain (EST1_DNA_bind) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCAAGCCCCCGCGACCAGCAGCTACCCG |
| Internal bar code: | CCGGAGGGACGGTAGGCTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.8517 |
| LEAP-Seq n confirming: | 3367 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGTCGCTACGGTGTCTT |
| Suggested primer 2: | ACAGCAAGCAGTTGGCTTTT |