| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207964 |
| Chromosome: | scaffold 24 |
| Location: | 49355 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755447 | (1 of 1) K12863 - protein CWC15 (CWC15) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCAGCAGGCGCCCCGCAGGGATCCCCAG |
| Internal bar code: | GTGGGCGAAAGGTAGTAGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 98.3871 |
| LEAP-Seq n confirming: | 427 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCCTTCTCTGCACCAG |
| Suggested primer 2: | ACTTCGTTTGAAGGTGGGTG |