Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.207964 |
Chromosome: | scaffold 24 |
Location: | 49574 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre24.g755447 | (1 of 1) K12863 - protein CWC15 (CWC15) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCTCCTCTCCAGTGCAGTACTGATGGA |
Internal bar code: | GGACGGACTGTTGCAGGGCAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 127 |
LEAP-Seq percent confirming: | 2.18905 |
LEAP-Seq n confirming: | 66 |
LEAP-Seq n nonconfirming: | 2949 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTTCGTTTGAAGGTGGGTG |
Suggested primer 2: | GCTACCCTTCTCTGCACCAG |