| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208001 |
| Chromosome: | chromosome 8 |
| Location: | 915035 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361600 | (1 of 3) PF10250 - GDP-fucose protein O-fucosyltransferase (O-FucT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAGGCTGGGCGTCTGTGCAAACGCAGC |
| Internal bar code: | ACGCCGATAGGGACGGTAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.1199 |
| LEAP-Seq n confirming: | 4730 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATGTTGAGCTATGACGA |
| Suggested primer 2: | AATGAAGGCGGGGATAGTTT |