| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208028 |
| Chromosome: | chromosome 12 |
| Location: | 8338524 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549500 | (1 of 2) 1.5.4.1 - Pyrimidodiazepine synthase | 3'UTR/3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAAACCCAGTTCGGGAGTGCCTATCAAA |
| Internal bar code: | ATACGCCCCCAAGGGTAAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 48.2206 |
| LEAP-Seq n confirming: | 271 |
| LEAP-Seq n nonconfirming: | 291 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTTGGGACTGCACTGTT |
| Suggested primer 2: | ACGGTTGATGTCTGGGCTAC |