| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208046 |
| Chromosome: | chromosome 5 |
| Location: | 174633 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234400 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAAAGTCGTGTCCACTGCAGCTCTGCG |
| Internal bar code: | GCTCGCATAAGTAGATAGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 97.8142 |
| LEAP-Seq n confirming: | 358 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTGTAAATGACCTGCTT |
| Suggested primer 2: | GATCTCCACACCCCAGAAGA |