| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208051 |
| Chromosome: | chromosome 10 |
| Location: | 799017 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423250 | MDN2,MDH2 | Malate dehydrogenase 2; (1 of 1) PTHR11540//PTHR11540:SF27 - MALATE AND LACTATE DEHYDROGENASE // MALATE DEHYDROGENASE, GLYOXYSOMAL-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGGAGCCCAGCAGCTCGGCCTGTGTGT |
| Internal bar code: | TTAGCACGTGGCGACCGTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTTAGTTAAGGCTCAGCG |
| Suggested primer 2: | GCTGTGGTAGAGGGAGGATG |