Insertion junction: LMJ.RY0402.208058_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.208058
Chromosome:chromosome 10
Location:5470944
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g458850 SRR32 Scavenger receptor cysteine rich (SRCR) protein; (1 of 20) IPR001190//IPR017448 - SRCR domain // SRCR-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTAGCACGGGTTGCGTGTATGTGTGCGGT
Internal bar code:CCGGAAGCCACGGCGTCGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:831
LEAP-Seq percent confirming:99.686
LEAP-Seq n confirming:2222
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GACCCACCGTGTAATCCATC
Suggested primer 2:GTACGAAATATGCACCGGCT