Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.208063 |
Chromosome: | chromosome_12 |
Location: | 3270303 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g498200 | FAP128 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACTTCGCCTTTTCCCGGGCAACATCTCCC |
Internal bar code: | GTGCAACGACCCCGTTACGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 704 |
LEAP-Seq percent confirming: | 99.7891 |
LEAP-Seq n confirming: | 6625 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACAATACGCCAAGACCCTG |
Suggested primer 2: | GCCTAGCCTACCCTCTGCTT |