| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208077 |
| Chromosome: | chromosome 17 |
| Location: | 3809764 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727801 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCACGACATGATGCAGGGGATGGATGGG |
| Internal bar code: | CGAGGTTGCGTTGCCGCCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 854 |
| LEAP-Seq percent confirming: | 99.3396 |
| LEAP-Seq n confirming: | 1805 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACCTGACCTGACAACCT |
| Suggested primer 2: | GACCTGTGGACGGAGTAGGA |