| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208081 |
| Chromosome: | chromosome 3 |
| Location: | 3062156 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164450 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCGTGCAGCTGCTGCCGAGCCGCCTCC |
| Internal bar code: | TCGTTCCCACGTGGGTCGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 60.8846 |
| LEAP-Seq n confirming: | 881 |
| LEAP-Seq n nonconfirming: | 566 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATCGGTACGCACAACAC |
| Suggested primer 2: | ACGTACATGCAACCTCACCA |