| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208089 |
| Chromosome: | chromosome 9 |
| Location: | 4072678 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393395 | (1 of 1) PTHR33477:SF3 - EMB|CAB72147.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGTGTTCGCTTAGTGACACCCGCATC |
| Internal bar code: | AGGACGATTTGCCAGGGGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 294 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGCGTTCATACAGGATT |
| Suggested primer 2: | TTACCGTACCAGATCCGAGG |