| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208100 |
| Chromosome: | chromosome 16 |
| Location: | 4924345 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGCAGCGGGTGTTTCGAGGGGCGGGCG |
| Internal bar code: | AGAGTGTGGTGGGCGATGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 32.0643 |
| LEAP-Seq n confirming: | 2552 |
| LEAP-Seq n nonconfirming: | 5407 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAACTCACCTAATCGCTC |
| Suggested primer 2: | TGTGAGGCTGGATAGTGTGC |