| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208112 |
| Chromosome: | chromosome 2 |
| Location: | 4246745 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098850 | NTR2 | NADPH dependent thioredoxin reductase 2; (1 of 2) PTHR22912//PTHR22912:SF183 - DISULFIDE OXIDOREDUCTASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACCTGCAGAAGCCACATGCGCAGTCTT |
| Internal bar code: | CGGGGTGAACCACGATCCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 99.359 |
| LEAP-Seq n confirming: | 620 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACGGGAAGAGACTGGAT |
| Suggested primer 2: | CGAAGCATATTTGCTCGTGA |