| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208147 |
| Chromosome: | chromosome 13 |
| Location: | 3294417 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586300 | FKB12 | Peptidyl-prolyl cis-trans isomerase, FKBP-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTGTCGCTCTCATCACAAGAGGCCACC |
| Internal bar code: | TGTTCTGCTCCGTGATGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 99.5004 |
| LEAP-Seq n confirming: | 3386 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGACTGGTGAGGGTAGGA |
| Suggested primer 2: | GCTACCCTGGTGTTCGATGT |