| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208170 |
| Chromosome: | chromosome 5 |
| Location: | 3111761 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239151 | (1 of 3) 6.3.2.25 - Tubulin--tyrosine ligase / TTL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTTGATAACGCGCGCCTGCCGGACGCT |
| Internal bar code: | CGTTGTGACCTTGGCGTTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 99.5098 |
| LEAP-Seq n confirming: | 8323 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGATGTGATGGCTGCTG |
| Suggested primer 2: | CCGGAGTGAAAAAGGTGAAA |