| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208189 |
| Chromosome: | chromosome 3 |
| Location: | 4055907 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172500 | PTO2,PTOX2 | (1 of 2) K17893 - ubiquinol oxidase (AOX1, AOX2); Plastid terminal oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAATTTGCACTGGTGCTGCAAGCTGCCG |
| Internal bar code: | GTGCATCGTCCGTGGTCTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 842 |
| LEAP-Seq percent confirming: | 99.6821 |
| LEAP-Seq n confirming: | 9719 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGAACTCCTGAACCCAC |
| Suggested primer 2: | AAGGCATCACAAATTCTCCG |