| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208192 |
| Chromosome: | chromosome 11 |
| Location: | 2527155 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475950 | HLM16 | putative N-methyltransferase; (1 of 5) IPR001214//IPR003616 - SET domain // Post-SET domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTTTAGGTCAATTCATGAAGCCACGGC |
| Internal bar code: | AGTGTGGATCATTCAGTATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 99.8847 |
| LEAP-Seq n confirming: | 866 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTGGCAGTTTGTCTCTT |
| Suggested primer 2: | CACATCCTAGCCCTGTGGTT |