Insertion junction: LMJ.RY0402.208199_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.208199
Chromosome:chromosome 13
Location:3733534
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTTGTGGACTGCTCGGGATCCATGGACG
Internal bar code:TCGTACTGACCAGGCGCTCTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:630
LEAP-Seq percent confirming:99.5972
LEAP-Seq n confirming:3462
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATCCACTCCTCCTCGTG
Suggested primer 2:CTCAGCAGTCTTCCGTTTCC