Insertion junction: LMJ.RY0402.208199_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.208199
Chromosome:chromosome 13
Location:3733539
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCCTTGCGCAGCTGCTGCTGCTTGCCG
Internal bar code:GTGTTCTCGCGTGGCAGAAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:709
LEAP-Seq percent confirming:98.6755
LEAP-Seq n confirming:149
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATCCACTCCTCCTCGTG
Suggested primer 2:CTCAGCAGTCTTCCGTTTCC