| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208200 |
| Chromosome: | chromosome 7 |
| Location: | 1649395 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGCGGCGCCTGCAGCCGCCGCTGCGGC |
| Internal bar code: | TGGTGAATTTAGTGCACCATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 780 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGGTCGTATTCACTGC |
| Suggested primer 2: | CGCAAGAGTTATGTCGCAAA |