| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208201 |
| Chromosome: | chromosome 9 |
| Location: | 6622272 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408600 | (1 of 1) 1.13.11.29 - Stizolobate synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAAGGCCAAGCAGCTCAGGGTGGCTA |
| Internal bar code: | GCATCGAAGCTTGACCGGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 99.809 |
| LEAP-Seq n confirming: | 3135 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTTATGGAAGGTGCGTG |
| Suggested primer 2: | TCTTGTGCGAGATGGTCTTG |