Insertion junction: LMJ.RY0402.208235_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.208235
Chromosome:chromosome 12
Location:1373211
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g488351 (1 of 1) IPR001368//IPR009030//IPR011641 - TNFR/NGFR cysteine-rich region // Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCCAAGCCCCTCTCCCAGCCCTTCACC
Internal bar code:GTCACTAAACCGTGACAGTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:33
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACGCAGTGCCTTGAGTA
Suggested primer 2:GCTGGGAGAGACATCTGGAG